Effects of tolC on tolerance to bile salts and biofilm formation in Cronobacter malonaticus

Bile salts is one of essential components bile secreted into the intestine to confer antibacterial protection. Cronobacter species are associated with necrotizing enterocolitis in newborns and show a strong tolerance salts. However, little attempt has been made focus on molecular basis In this study, we investigated roles tolC growth, cell morphology, motility, biofilm formation ability malonaticus under salt stress. The results indicated that absence significantly affected colony morphology outer membrane structure normal situation, compared those wild type strain. deletion caused decline resistance stress, inhibition observable reduction relative growth rate motility. Moreover, bacterial stress response promoted mutant expression AcrAB-TolC system (acrA, acrB, tolC) was effectively upregulated control sample when exposed different concentrations. findings provide valuable information for deeply understanding mechanisms about prevention C. malonaticus.